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腹腔灌洗液中低密度中性粒细胞分泌的中性粒细胞胞外陷阱介导肿瘤细胞生长与附着
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Cancer Research
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JoVE Journal Cancer Research
Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment
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腹腔灌洗液中低密度中性粒细胞分泌的中性粒细胞胞外陷阱介导肿瘤细胞生长与附着

DOI:
10.3791/58201-v

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08:59 min

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August 03, 2018

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, , , , , , ,
1Department of Gastrointestinal Surgery,Jichi Medical University

Chapters

  • 00:04Title
  • 00:57Low Density Neutrophil (LDN) Abdominal Cavity Lavage Isolation
  • 03:44Neutrophil Extracellular Trap (NET) Detection and Tumor Cell Adhesion Analysis
  • 06:37Results: Representative LDN: Tumor Cell Attachment Analyses
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出一种方法, 其中人类低密度中性粒细胞 (LDN), 从术后腹腔灌洗液中恢复, 产生大量的中性粒细胞外圈闭 (网), 并有效地诱捕后生长的游离肿瘤细胞。

Tags

Neutrophil Extracellular TrapsNETsLow Density NeutrophilsPeritoneal Lavage FluidTumor Cell GrowthTumor MetastasisNeutrophil SubpopulationsTumor ImmunologyDensity Gradient SeparationMagnetic Activated Cell SortingMACSCD66bGastrointestinal Malignancy

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