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Cancer Research
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c-fos 和 dusp1 在癌基因依赖性中的作用评价方法
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Cancer Research
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JoVE Journal
Cancer Research
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
Please note that all translations are automatically generated.
Click here for the English version.
c-fos 和 dusp1 在癌基因依赖性中的作用评价方法
DOI:
10.3791/58194-v
•
10:09 min
•
January 07, 2019
•
Meenu Kesarwani
,
Zachary Kincaid
,
Mohammad Azam
1
Cancer Blood Disease Institute, Divisions of Experimental Hematology and Cancer Pathology
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:04
Title
00:48
Isolation of c-Kit+ Cells from Bone Marrow
03:52
Transduction
06:43
Colony-forming Unit Assays
08:25
Results: c-Fos and Dusp1 Therapeutic Target in Leukemia
09:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了 c-fos 和 dusp1 作为白血病药物靶标的遗传和化学验证协议, 在体外和体内使用遗传和人性化的小鼠模型。该方法可应用于遗传验证和治疗发展的任何目标。
Tags
C-Fos
Dusp1
Oncogene Dependence
Cancer Therapeutics
Leukemia
Solid Tumors
Lung Cancer
Brain Tumors
Pancreatic Tumors
Bone Marrow
Mouse
Cell Isolation
CD117
MACS
Cell Separation
Cell Culture
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