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单细胞荧光显微镜定量 Efferocytosis 的研究

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Immunology and Infection

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JoVE Journal Immunology and Infection

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

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单细胞荧光显微镜定量 Efferocytosis 的研究

DOI:

10.3791/58149-v

•

06:15 min

•

August 18, 2018

•

Kyle Taruc, Charles Yin, Daniel G. Wootton³, Bryan Heit

¹Department of Microbiology and Immunology and the Center for Human Immunology,University of Western Ontario, ²Institute of Infection and Global Health,University of Liverpool, ³Department of Respiratory Research,Aintree University Hospital NHS Foundation Trust

Chapters

00:04Title
00:50Quantifying Efferocytic Uptake and Dynamics Using a Fixed Cell Efferocytosis Assay and Inside-out Staining
05:14Results: Overnight Culture of Jurkat Cells with Staurosporine Results in Apoptosis of Greater than 95% of Cells
05:36Conclusion

Summary

Automatic Translation

Efferocytosis, 凋亡细胞的吞噬去除, 是需要保持动态平衡, 并由受体和信号通路, 以允许识别, 吞没和内化的凋亡细胞的促进。在此, 我们提出了一个荧光显微镜协议的量化 efferocytosis 和 efferocytic 信号通路的活动。

Tags

Efferocytosis Single-cell Fluorescence Microscopy Apoptotic Cell Uptake Macrophages Jurkat Cells NHS-Biotin Cell-tracking Dye FITC-conjugated Streptavidin Quantification Microscope Optimization

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