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SA-β-加拉基沙酶筛查检测,用于识别治疗药物
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SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
Please note that all translations are automatically generated.
Click here for the English version.
SA-β-加拉基沙酶筛查检测,用于识别治疗药物
DOI:
10.3791/58133-v
•
07:39 min
•
June 28, 2019
•
Heike Fuhrmann-Stroissnigg*
1
,
Fernando E. Santiago*
1,2,3
,
Diego Grassi
,
YuanYuan Ling
,
Laura J. Niedernhofer
2,3
,
Paul D. Robbins
2,3
1
Department of Molecular Medicine and the Center on Aging
,
The Scripps Research Institute
,
2
Department of Biochemistry, Molecular Biology and Biophysics
,
University of Minnesota
,
3
Institute on the Biology of Aging and Metabolism
,
University of Minnesota
Chapters
00:04
Title
01:06
Generation of Senescent Murine Embryonic Fibroblast (MEF) 12-15 Days
03:46
Senescent Associated -Galactosidase (SA–Gal) Screening Assay 2-3 Days
06:06
Results: Identification of Senotherapeutics by Measuring SA–Gal Activity in Single Cells
06:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞衰老是慢性年龄相关疾病发展的关键因素。针对衰老细胞的治疗药物的鉴定为延长健康衰老提供了希望。在这里,我们提出了一个新的测定,以筛选基于测量与衰老相关的β-加拉托西沙酶在单细胞中的异质疗法的识别。
Tags
SA-β-Galactosidase
Senotherapeutic Drugs
Senolytic
Senomorphic
High-throughput Drug Screening
Aging-related Diseases
Cell Senescence
Cell Culture
Trypsinization
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