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Journal
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Immunology and Infection
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心脏外免疫细胞的分离与鉴定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation and Identification of Extravascular Immune Cells of the Heart
Please note that all translations are automatically generated.
Click here for the English version.
心脏外免疫细胞的分离与鉴定
DOI:
10.3791/58114-v
•
08:24 min
•
August 23, 2018
•
Laura Aronoff
2
,
Slava Epelman
2,3,4,5
,
Xavier Clemente-Casares
2
1
Toronto General Hospital Research Institute
,
University Health Network (UHN)
,
2
Dept of Laboratory Medicine and Pathobiology
,
University of Toronto
,
3
Dept of Immunology
,
University of Toronto
,
4
Peter Munk Cardiac Centre
,
5
Ted Rogers Centre for Heart Research
Chapters
00:04
Title
00:40
Circulating Leukocyte Labeling and Analysis
06:22
Results: Cardiac Antigen Presenting Cell Quantification
07:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议提出了一种简便、有效的方法, 用于分离、鉴定和量化在小鼠心肌中的免疫细胞在稳态或炎症状态下的存在。该协议结合酶和机械消化的生成单细胞悬浮, 可以进一步分析的流式细胞术。
Tags
Immune Cells
Heart
Immunophenotyping
Cardiac Immune Cell Populations
Tail Vein Injection
Fluorophore-conjugated Anti-CD45 Antibody
Heart Perfusion
Intravascular Contaminants
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