A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:55Mouse Mesenteric Artery Dissection
- 02:26Arterial Mounting
- 05:30Normalization
- 07:03Artery Contraction Recording
- 08:18Results: Representative Tracings Showing the Normalization Procedure, Potentiation by High-K+ Solution, and Contraction
- 08:47Conclusion
A técnica de myograph do fio é usada para investigar as funções do músculo liso vascular e tela novas drogas. Nós relatamos um protocolo detalhado para medir a contratilidade isométrica da artéria mesentérica de rato e para a seleção de novos relaxantes do músculo liso vascular.
Tags
Isometric ContractilityWire MyographyMesenteric ArteryVascular Smooth MuscleContractility MeasurementHypertension TherapyHigh PotassiumMulti-channel SystemDrug ScreeningEuthanized MouseGastrointestinal TractMesenteric Artery DissectionHEPES-Tyrode SolutionStereoscopic MicroscopeCalcium-free SolutionArtery SegmentStainless Steel Wire
