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Journal
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Neuroscience
/
果蝇
幼虫和成年脑的代谢分析
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Metabolic Analysis of
Drosophila melanogaster
Larval and Adult Brains
Please note that all translations are automatically generated.
Click here for the English version.
果蝇
幼虫和成年脑的代谢分析
DOI:
10.3791/58007-v
•
07:06 min
•
August 07, 2018
•
Kathryn E. Neville
,
Timothy L. Bosse
,
Mia Klekos
,
John F. Mills
,
Marla Tipping
1
Department of Biology
,
Providence College
Chapters
00:04
Title
00:40
Preparation of the Micro-tissue Restraints
01:23
Dissection of the
Drosophila melanogaster
Larval Brains
02:44
Addition of the Dissected Brains and Micro-tissue Restraints to the 96-well Metabolic Assay Cell Plate
04:52
Addition of the Cell Plate to the Metabolic Analyzer and the Post-measurement Analysis
05:37
Results: The OCR and ECAR Measurements in Adult and Larval
Drosophila melanogaster
Brains
06:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文提出了一种测定
果蝇
幼虫和成年脑中氧耗量和细胞外酸化的方法。代谢分析仪是利用一个适应和优化的协议。微组织约束是本协议的重要组成部分, 专门为其在分析中的应用而设计和创建。
Tags
Drosophila Melanogaster
Larval Brain
Adult Brain
Metabolic Analysis
Metabolic Reprogramming
Glial Cells
Diet
Behavior
Imaginal Discs
C. Elegans
Dissection
96-well Metabolic Assay Plate
Assay Media
PBS
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