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Journal
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Immunology and Infection
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快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples
Please note that all translations are automatically generated.
Click here for the English version.
快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒
DOI:
10.3791/58001-v
•
06:59 min
•
June 30, 2018
•
Maiken W. Rosenstierne
,
Christopher E. Jensen
,
Anders Fomsgaard
2
1
Virus Research & Development Laboratory
,
Statens Serum Institut
,
2
Infectious Disease Research Unit
,
University of Southern Denmark
Chapters
00:04
Title
00:44
Preparation for Virus Inactivation and Rapid Nucleic Acid Extraction
02:02
Blood Collection from Patients with Signs and Symptoms of a Virus Infection
02:50
Simplified Nucleic Acid Extraction from Whole Blood
05:04
Results: Sensitivity of the Simplified NA Extraction Method
06:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个快速病毒核酸提取从病毒灭活全血的协议。萃取是直接在采血管, 不需要设备或电力。该方法不依赖于实验室设施, 可以在任何地方使用 (
例如
在野战医院)。
Tags
Manual Bedside Nucleic Acid Extraction
Virus Detection
Whole Blood Samples
Point-of-care Diagnostics
Blood Collection
Magnetic Glass Particles
Wash Buffers
Elution Buffer
Virus Inactivation
Nucleic Acid Purification
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