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Journal
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Immunology and Infection
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全血流式细胞术分析人单核细胞亚群的表征
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Please note that all translations are automatically generated.
Click here for the English version.
全血流式细胞术分析人单核细胞亚群的表征
DOI:
10.3791/57941-v
•
09:12 min
•
October 17, 2018
•
Rekha Marimuthu
2
,
Habib Francis
2
,
Suat Dervish
,
Stephen C.H. Li
,
Heather Medbury*
1,2
,
Helen Williams*
1,2
1
Department of Surgery, Vascular Biology Research Centre
,
Westmead Hospital
,
2
Westmead Clinical School, Department of Surgery
,
The University of Sydney
,
3
Westmead Research Hub
,
Westmead Institute for Medical Research
,
4
Institute for Clinical Pathology and Medical Research
,
Westmead Hospital
Chapters
00:04
Title
00:42
Sample Preparation for Whole Blood Flow Cytometry
02:14
Flow Cytometry
03:28
Monocyte Gating
07:11
Results: Validation of Gating and Quantification of Marker Expression
08:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个通过全血流式细胞仪表征单核细胞子集的协议。这包括概述如何门的子集和评估他们的表面标记的表达, 并提供了一个例子, 评估 M1 (炎症) 和 M2 标记 (抗炎) 的表达。
Tags
Monocyte Subsets
Flow Cytometry
Whole Blood Analysis
CD14
CD16
HLA-DR
M1 And M2 Markers
Gating
Red Blood Cell Lysis
White Blood Cell Fixation
Sample Preparation
Flow Cytometry Setup
Quality Control
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