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就地石蜡镶嵌成人珊瑚样品的杂交技术
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Environment
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JoVE Journal Environment
In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
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就地石蜡镶嵌成人珊瑚样品的杂交技术

DOI:
10.3791/57853-v

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07:24 min

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August 31, 2018

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1Department of Marine Biology and Ecology,University of Miami, Rosenstiel School of Marine and Atmospheric Sciences

Chapters

  • 00:04Title
  • 00:52Removal of Paraffin
  • 01:44Pretreatment of Slides for Preparation of RNA Probe Hybridization
  • 03:03Hybridization of the RNA Probe
  • 04:07Visualization of the RNA Probe
  • 06:18Results: Adult Corals Exposed to Heat Stress Show Spatial Expression of RNA Probes
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

本议定书的目的是对已嵌入石蜡切片的成人珊瑚样品进行原位杂交, 并将其切片到玻璃滑梯上。这是一种定性的方法, 用于可视化的 RNA 反感探针在石蜡嵌入组织的空间表达。

Tags

In Situ HybridizationParaffin-embeddedAdult Coral SamplesCoral Cell BiologyGene ExpressionSpatial Gene ExpressionInvertebrate SystemsDewaxingEthanol GradientProteinase KGlycine PBSSaline Sodium Citrate (SSC)Hybridization BufferProbe DilutionHybridization Oven

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