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Journal
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Immunology and Infection
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细胞法检测酶替代疗法中细胞吸收的抗体
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay
Please note that all translations are automatically generated.
Click here for the English version.
细胞法检测酶替代疗法中细胞吸收的抗体
DOI:
10.3791/57777-v
•
07:52 min
•
September 10, 2018
•
Ruby Cheung
,
Gregory W. deHart
,
Lynne Jesaitis
,
Stephen J. Zoog
,
Andrew C. Melton
1
BioMarin Pharmaceutical Inc
Chapters
00:04
Title
00:56
Cell Plating and Sample Preparation
03:43
Sample Addition and Cell Viability Staining
05:44
Results: Representative Neutralizing Antibody (NAb) Detection by Enzyme Replacement Therapy (ERT)
07:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一种基于细胞的流式细胞仪方法来检测中和抗体或其他因素, 干扰细胞吸收酶替代疗法在人类基质, 如脑脊髓液 (CSF) 或人血清。
Tags
Antibodies
Neutralizing
Enzyme Replacement Therapy
Cell-based Assay
Immunogenicity
Bioanalytical
Drug Safety
Efficacy
Pharmacokinetics
Pharmacodynamics
Jurkat Cells
Mannose-6-phosphate Receptor
Flow Cytometry
ERT Beads
Magnetic Separation
Rotary Shaker
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