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Cancer Research
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GPC3-targeting 双特异性抗体, GPC3-S-Fab, 具有强大的细胞毒性
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A GPC3-targeting Bispecific Antibody, GPC3-S-Fab, with Potent Cytotoxicity
Please note that all translations are automatically generated.
Click here for the English version.
GPC3-targeting 双特异性抗体, GPC3-S-Fab, 具有强大的细胞毒性
DOI:
10.3791/57588-v
•
11:13 min
•
July 12, 2018
•
Yanlan Wang
2
,
Jiayu Liu
2
,
Haitao Pan
2
,
Jieyu Xing
2
,
Xiaoqiong Wu
2
,
Qing Li
2
,
Zhong Wang
2
1
School of Pharmaceutical Sciences
,
Sun Yat-Sen University
,
2
Center for Cellular & Structural Biology
,
Sun Yat-Sen University
Chapters
00:04
Title
00:40
GPC3-S-Fab Periplasmic Purification
04:00
Cytotoxic Assay
08:32
Results: Study of the Construction and Functional Importance of Bispecific Antibody GPC3-S-Fab
10:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在此, 我们提出了一种在大肠杆菌中产生双特异性抗体 GPC3-S-Fab 的协议
.
纯化 GPC3-S-Fab 对 GPC3 阳性肝癌细胞具有较强的细胞毒性作用。
Tags
Bispecific Antibody
GPC3-S-Fab
E. Coli
Periplasmic Fraction
Nickel-NTA Agarose
Cytotoxicity
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