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1David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine,The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine,University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine,The University of Tokyo
Chapters
- 00:04Title
- 00:41Induction of EndMT
- 01:58Immunocytochemical Analysis
- 05:02Three-dimensional Collagen Gel Contraction Assay
- 06:09Results: Use of an In Vitro Method to Study Endothelial-mesenchymal Transition Upon TGF-β Treatment
- 07:20Conclusion
Se describe un protocolo para la inducción en vitro de transición endotelio-mesénquima (EndMT), que es útil para la investigación de vías de señalización celulares involucradas en EndMT. En este modelo experimental, EndMT es inducida por el tratamiento con TGF-β en las células endoteliales MS-1.
