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深部真皮注射液作为白色念珠菌皮肤感染模型的组织学分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses
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深部真皮注射液作为白色念珠菌皮肤感染模型的组织学分析

DOI:
10.3791/57574-v

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10:45 min

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June 13, 2018

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, , , ,
1Department of Biotechnology and Biosciences,University of Milano-Bicocca, 2Harvard Medical School and Division of Gastroenterology,Boston Children’s Hospital

Chapters

  • 00:05Title
  • 00:54C. albicans Preparation
  • 02:29Deep Dermal Injection
  • 03:57Sample Collection and Preparation for Histological Staining and Slides
  • 05:09Hematoxin & Eosin Staining
  • 06:07Periodic Acid-Schiff Staining
  • 07:19Sample Collection and Preparation for RNA Extraction
  • 08:30Results: Histological Analysis of C. albicans Infection in Mice
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个协议, 允许皮肤标本的组织学和分子分析后,白色念珠菌皮注射液。该协议维护皮肤的结构完整性, 并允许组织居民或新招募的免疫细胞的定位以及病原体的分布。

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