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一种研究外周和中枢神经系统 Neuroregeneration 的果蝇体内损伤模型
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A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System
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一种研究外周和中枢神经系统 Neuroregeneration 的果蝇体内损伤模型

DOI:
10.3791/57557-v

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09:55 min

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May 05, 2018

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, , ,
1Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine,University of Pennsylvania

Chapters

  • 00:04Title
  • 01:08Collecting Larvae
  • 02:11Microscope Set Ups
  • 04:18Two-photon Injury
  • 07:40Results: Axon Regeneration in the PNS
  • 08:49Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个协议使用果蝇感觉神经元-树突状树枝状 (da) 神经元损伤模型, 结合在体内活成像, 双光子激光切断/dendriotomy, 和强大的苍蝇遗传工具箱, 作为neuroregeneration 的潜在促进剂和抑制剂的筛选平台。

Tags

DrosophilaIn Vivo Injury ModelNeuroregenerationPeripheral Nervous SystemCentral Nervous SystemLive ImagingTwo-photon Laser Axotomy/dendriotomyGenetic ToolboxScreeningAxon RegenerationDendritic RegenerationNeurodegenerative DiseasesNeuron-glia InteractionsLarval CollectionCulture BottlesGrape Juice Agar PlateImagingTwo-photon MicroscopeGFPLaser IntensityPNS InjuryVNC Injury

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