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Journal
/
Immunology and Infection
/
分離と大人
住血吸虫
から細胞外小胞のキャラクタリゼーション
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation and Characterization of Extracellular Vesicles from Adult
Schistosoma japonicum
Please note that all translations are automatically generated.
Click here for the English version.
分離と大人
住血吸虫
から細胞外小胞のキャラクタリゼーション
DOI:
10.3791/57514-v
•
07:37 min
•
May 22, 2018
•
Juntao Liu*
1
,
Lihui Zhu*
1
,
Lihui Wang
2
,
Yongjun Chen
,
Bikash Ranjan Giri
,
Jianjun Li
,
Guofeng Cheng
1
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology
,
Ministry of Agriculture
,
2
Tianjin Agricultural University
Chapters
00:04
Title
00:46
Isolating SjEVs from
Schistosoma japonicum
Cultures
03:00
Characterization of EVs by Western Blotting and TEM
04:01
Labeling
Schistosoma japonicum
Extracellular Vesicles with PKH67 Followed by Cell Uptake
06:15
Results:
Schistosoma japonicum
Extracellular Vesicle Characterization
07:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、アダルト
住血吸虫
から培養
体外
細胞小胞 (Ev) の分離を記述するプロトコルを提案する.
Tags
Extracellular Vesicles
Schistosoma Japonicum
In Vitro Culture
Isolation
Purification
Centrifugation
Dialysis
PEG8000
Western Blot
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