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Journal
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Cancer Research
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3D 脊髓黑色素瘤球形皮肤模型
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
A 3D Organotypic Melanoma Spheroid Skin Model
Please note that all translations are automatically generated.
Click here for the English version.
3D 脊髓黑色素瘤球形皮肤模型
DOI:
10.3791/57500-v
•
08:49 min
•
May 18, 2018
•
Ines Müller
,
Dagmar Kulms
1
Experimental Dermatology, Medical Faculty
,
TU-Dresden
Chapters
00:04
Title
01:20
Generation of 3D Melanoma Spheroids via the Hanging Drop Method
03:16
Generation of the Dermal and Epidermal Counterparts of Organotypic Full Skin Reconstructs
05:08
Air-liquid Cultivation of Organotypic Full Skin Reconstructs and Generation of Organotypic Melanoma Spheroid Skin Reconstructs
06:45
Results: Validation of Organotypic Full Skin Reconstruct and Melanoma Spheroid Skin Reconstruct
08:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出一个协议来生成一个3D 脊髓黑色素瘤球形皮肤模型, 概括的结构和多细胞复杂性的器官/肿瘤
在体内
, 但同时容纳系统实验干预。
Tags
3D Organotypic Melanoma Spheroid Skin Model
Tumor-host Interaction
Therapeutic Intervention
3D Architecture
Non-vascularized Metastases
Tumor Heterogeneity
Primary Cells
Melanoma Cells 451LU
Melanoma Spheroids
Hanging Drop
Skin Reconstructs
Dermal Counterpart
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