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人鼻上皮细胞球体的生成与个体化囊性纤维膜电导调节的研究
JoVE Journal
Medicine
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JoVE Journal Medicine
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
DOI:

08:00 min

April 11, 2018

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Chapters

  • 00:04Title
  • 00:41Passage Human Nasal Epithelial Cells
  • 02:45Seeding Cells for HNE Spheroid Cultures
  • 04:37Culture Differentiated and Mature HNE Spheroids
  • 05:27Results: Individualized CFTR Study Through the Generation of HNE Spheroids
  • 07:01Conclusion

Summary

Automatic Translation

这里我们描述了一种生成三维的人鼻上皮细胞的球形培养方法。球体被刺激以驱动囊性纤维化跨膜电导调节剂 (CFTR) 依赖的离子和液体分泌物, 将球体腔内大小的变化量化为 CFTR 功能的代表。

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