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人鼻上皮细胞球体的生成与个体化囊性纤维膜电导调节的研究
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Medicine
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JoVE Journal
Medicine
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
Please note that all translations are automatically generated.
Click here for the English version.
人鼻上皮细胞球体的生成与个体化囊性纤维膜电导调节的研究
DOI:
10.3791/57492-v
•
08:00 min
•
April 11, 2018
•
John J. Brewington
,
Erin T. Filbrandt
,
Francis J. LaRosa III
,
Jessica D. Moncivaiz
,
Alicia J. Ostmann
,
Lauren M. Strecker
,
John P. Clancy
1
Department of Pediatrics, Division of Pulmonary Medicine
,
Cincinnati Children’s Hospital Medical Center
Chapters
00:04
Title
00:41
Passage Human Nasal Epithelial Cells
02:45
Seeding Cells for HNE Spheroid Cultures
04:37
Culture Differentiated and Mature HNE Spheroids
05:27
Results: Individualized CFTR Study Through the Generation of HNE Spheroids
07:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里我们描述了一种生成三维的人鼻上皮细胞的球形培养方法。球体被刺激以驱动囊性纤维化跨膜电导调节剂 (CFTR) 依赖的离子和液体分泌物, 将球体腔内大小的变化量化为 CFTR 功能的代表。
Tags
Nasal Epithelial Cell Spheroids
CFTR Studies
Cystic Fibrosis
Ex Vivo Model
Cell Culture
Trypsinization
Cell Expansion
Cell Differentiation
Basement Membrane Matrix
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