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Biology
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从兔模型中分离纯化滑液源性间充质干细胞的磁活化细胞分选策略
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Biology
Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model
Please note that all translations are automatically generated.
Click here for the English version.
从兔模型中分离纯化滑液源性间充质干细胞的磁活化细胞分选策略
DOI:
10.3791/57466-v
•
10:20 min
•
August 10, 2018
•
Zhaofeng Jia
2,3
,
Yujie Liang
5
,
Xingfu Li
3
,
Xiao Xu
2,3
,
Jianyi Xiong
3
,
Daping Wang
2,3
,
Li Duan
3
1
Postgraduate institution
,
Guangzhou Medical University
,
2
Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology
,
3
Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopaedic Engineering
,
Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University)
,
4
Department of Chemistry
,
Chinese University of Hong Kong
,
5
Shenzhen Kangning Hospital
,
Shenzhen Mental Health Center
Chapters
00:04
Title
01:07
Isolate and Culture the rbSF-MSCs
03:21
CD90-positive Magnetic Activated Cell Sorting (MACS) of the rbSF-MSCs and Primary Culture
05:01
Magnetic Separation and Culture of te CD90
+
rbSF-MSCs
06:44
Results: Obtain Purified Rabbit SF-MSCs by CD90-Based Magnetic Activated Cell Sorting
08:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文提出了一种简单而经济的协议, 直接隔离和纯化新西兰白兔滑液间充质干细胞。
Tags
Magnetic-activated Cell Sorting
Mesenchymal Stem Cells
Synovial Fluid
Rabbit Model
Isolation
Purification
FDA-approved
Cell Culture
Cell Suspension
Centrifugation
Cell Strainer
Cell Colonies
Cell Passaging
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