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激光捕获显微切割液相色谱质谱 (LCM-LC/MS) 对肺结核患者药物的空间量化
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JoVE Journal Medicine
Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS)
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激光捕获显微切割液相色谱质谱 (LCM-LC/MS) 对肺结核患者药物的空间量化

DOI:
10.3791/57402-v

•

09:58 min

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April 18, 2018

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, , , ,
1Public Health Research Institute, New Jersey Medical School,Rutgers, The State University of New Jersey

Chapters

  • 00:04Title
  • 01:00Tissue Sectioning
  • 02:55Microdissection
  • 06:20Extraction and LC/MS Analysis
  • 08:14Results: Spatially-resolved Quantification of Ethambutol in Pulmonary Lesions by LCM-LC/MS
  • 09:19Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个协议使用激光捕获显微切割结合 LC/MS 分析, 以空间量化的药物分布在肺结核肉芽肿。该方法具有广泛的适用性, 以量化在高空间细节组织内的药物浓度。

Tags

Spatial QuantificationLaser Capture MicrodissectionLiquid Chromatography Mass SpectrometryLCM-LC/MSTuberculosisPulmonary LesionsEthambutolVisual DemonstrationCryostatOptimal Cutting Temperature AdhesivePET Membrane SlidesThaw-mountingAir-drying

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