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rna 干扰的实际应用: 口服双链 rna 在脂质体载体为蟑螂
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Biology
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JoVE Journal
Biology
Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
Please note that all translations are automatically generated.
Click here for the English version.
rna 干扰的实际应用: 口服双链 rna 在脂质体载体为蟑螂
DOI:
10.3791/57385-v
•
08:26 min
•
May 01, 2018
•
Jia-Hsin Huang
,
Yun Liu
,
Yu-Hsien Lin
2,3
,
Xavier Belles
,
How-Jing Lee
1
Department of Entomology
,
National Taiwan University
,
2
Biology Centre, Institute of Entomology
,
Czech Academy of Sciences
,
3
Faculty of Science
,
University of South Bohemia
,
4
Institute of Evolutionary Biology, CSIC-UPF
Chapters
00:04
Title
00:50
Collection of Extracellular Enzymes from Hemolymph
02:13
Collection of Extracellular Enzymes from Midgut Juice
03:31
dsRNA Degradation Assay
05:31
Oral Administration of dsRNA
06:42
Results: Knock Down of Tubulin Expression in the Midgut
07:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿通过口服双链 RNA 包裹在脂质体中, 证明了德国蟑螂中肠内基因表达的耗竭。
Tags
RNA Interference
Double-stranded RNA
Liposome Carriers
Cockroaches
Pest Control
Gene Silencing
Hemolymph Collection
Midgut Juice
Insect Saline Buffer
Protein Quantification
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