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Journal
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Immunology and Infection
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基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells
Please note that all translations are automatically generated.
Click here for the English version.
基于聚丙烯酰胺的多井格式实验研究细胞外基质硬度对黏附细胞细菌感染的影响
DOI:
10.3791/57361-v
•
11:51 min
•
July 05, 2018
•
Effie E Bastounis
,
Fabian E Ortega
,
Ricardo Serrano
,
Julie A Theriot
1
Department of Biochemistry
,
Stanford University School of Medicine
,
2
Department of Mechanical and Aerospace Engineering
,
University of California San Diego
,
3
Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute
,
Stanford University School of Medicine
Chapters
00:05
Title
00:39
Manufacturing Thin Two-layered Polyacrylamide Hydrogels on Multi-well Plates
01:36
Polyacrylamide Hydrogel Fabrication
05:22
Infection of Human Microvascular Endothelial Cells with L.
monocytogenes
07:11
Flow Cytometry to Quantify Extracellular-matrix-stiffness Dependent Susceptibility of Host Cells to Infection
08:02
Quantitative Time-lapse Microscopy to Assess Extracellular-matrix-stiffness Dependent
L. monocytogenes
Dissemination Through Endothelial Cells
09:01
Results: The Effect of Extracellular Matrix Stiffness on
L. monocytogenes
Infection of HMEC-1 Cells
10:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们开发了一种多井格式的聚丙烯酰胺检测方法, 以探讨细胞外基质硬度对黏附细胞细菌感染的影响。该检测与流式细胞术、染色和牵引力显微镜相兼容, 可以定量测量细胞、胞外基质和致病细菌之间的生物力学相互作用。
Tags
Polyacrylamide
Extracellular Matrix
Stiffness
Bacterial Infection
Adherent Cells
Host-pathogen Biomechanics
Hydrogels
Acrylamide
Bis-acrylamide
TEMED
APS
Fluorescent Microbeads
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