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Journal
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Immunology and Infection
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具有量身定做多样性的合成噬菌体展示库的构建
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Please note that all translations are automatically generated.
Click here for the English version.
具有量身定做多样性的合成噬菌体展示库的构建
DOI:
10.3791/57357-v
•
12:31 min
•
May 01, 2018
•
Ganggang Huang*
1
,
Zhenwei Zhong*
1
,
Shane Miersch
3
,
Sachdev S. Sidhu
2,3
,
Shin-chen Hou
,
Donghui Wu
1
Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies
,
ShanghaiTech University
,
2
Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research
,
University of Toronto
,
3
Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research
,
University of Toronto
Chapters
00:04
Title
01:18
Electroporation and Calculation of the Library Size
06:02
Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
09:43
Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
11:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本议定书描述了一个详细的程序, 以构建一个噬菌体显示合成抗体库, 具有量身定做的多样性。合成抗体具有广泛的应用, 从基础研究到疾病诊断和治疗。
Tags
Synthetic Phage Display
Fab Library
Antibody Library Construction
Antibody Diversity
Monoclonal Antibody Development
Phage Display Technology
Kunkel Mutagenesis
Electroporation
Diversity Evaluation
ELISA
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