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Journal
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Immunology and Infection
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胆汁盐诱导肠道病原菌生物膜形成的鉴别和定量技术
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
Please note that all translations are automatically generated.
Click here for the English version.
胆汁盐诱导肠道病原菌生物膜形成的鉴别和定量技术
DOI:
10.3791/57322-v
•
10:05 min
•
May 06, 2018
•
Kourtney P. Nickerson
2
,
Christina S. Faherty
2
1
Mucosal Immunology and Biology Research Center, Division of Pediatric Gastroenterology and Nutrition
,
Massachusetts General Hospital
,
2
Department of Pediatrics
,
Harvard Medical School
Chapters
00:04
Title
01:08
Solid-phase Adherence Assay
03:55
Extracellular Polymeric Substance Matrix Detection
05:20
Dispersion Assay
07:07
Results: Use of Solid-phase Adherence and Extracellular Polymeric Substance Matrix Formation to Detect Biofilm Formation and Dispersion
08:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议使读者能够分析胆汁盐诱导的肠道致病菌的生物膜形成, 采用多层面的方法来通过评估附着体、胞外高分子物质基质形成来捕获细菌生物膜的动态特性,和分散。
Tags
Bile Salts
Biofilm Formation
Enteric Pathogens
Bacterial Pathogenesis
Shigella Flexneri
Crystal Violet Assay
Quantification
96-well Plate
Static Incubation
PBS Washing
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