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直立活体显微术制备小鼠颌下腺涎腺
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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直立活体显微术制备小鼠颌下腺涎腺

DOI:
10.3791/57283-v

•

08:13 min

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May 07, 2018

•

, , ,
1Theodor-Kocher Institute,University of Bern, 2Center for Infectious Diseases, Integrative Virology,University Clinic of Heidelberg

Chapters

  • 00:05Title
  • 00:39Removal of the Fur and Fixation of the Mouse on the Stage
  • 02:46Surgical Exposure of the Salivary Gland Using a Stereomicroscope
  • 04:09Fixing of the Gland, Heating Ring, and the Temperature Probe
  • 06:10Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一个协议, 以手术揭露和稳定的小鼠颌下腺腺活体成像使用直立活体显微镜。该协议很容易适应小鼠和其他小啮齿目动物头部和颈部区域外分泌腺体。

Tags

Murine Submandibular Salivary GlandUpright Intravital MicroscopySurgical PreparationImmunologyC57 Black Six MouseHair Removal CreamCoverslip HolderEar BarSurgical TapeAngled Curved ForcepsHeating LampSubmandibular Salivary Gland

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