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Biology
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大豆原生质体分离的简易方法及其在瞬态基因表达分析中的应用
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Biology
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JoVE Journal
Biology
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Please note that all translations are automatically generated.
Click here for the English version.
大豆原生质体分离的简易方法及其在瞬态基因表达分析中的应用
DOI:
10.3791/57258-v
•
09:22 min
•
January 25, 2018
•
Faqiang Wu
,
Yoshie Hanzawa
1
Department of Biology
,
California State University, Northridge
Chapters
00:05
Title
00:57
Protoplast Isolation
04:09
Protoplast Transformation
06:00
Protoplast Incubation and Harvesting
06:46
Results: Efficient Isolation of Soybean Protoplasts for Transient Gene Expression Studies
08:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们开发了一个简单和有效的协议, 以制备大量的大豆原生质体研究复杂的调控和信号机制的活细胞。
Tags
Soybean Protoplasts
Transient Gene Expression
Regulatory Networks
Protein Interactions
Unifoliate Leaves
Enzyme Solution
Vacuum Infiltration
Protoplast Digestion
W5 Solution
Nylon Mesh
Centrifugation
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