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微生物菌落生物膜的石蜡嵌入和薄切片显微分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
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微生物菌落生物膜的石蜡嵌入和薄切片显微分析

DOI:
10.3791/57196-v

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08:52 min

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March 23, 2018

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, , , , ,
1Department of Biological Sciences,Columbia University, 2Department of Biology, Barnard College,Columbia University

Chapters

  • 00:04Title
  • 00:49Growth of Pseudomonas aeruginosa Colony Biofilms and Overlaying Colonies with Agar
  • 02:33Fixation and Sample Processing
  • 03:02Sample Embedding and Sectioning
  • 05:23Heat Fixing, Rehydration, and Mounting
  • 06:48Results: Microscopic Analysis of Microbial Colony Biofilms
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了微生物菌落生物膜的固定、石蜡嵌入和薄切片技术。在制备的样品中, 生物膜子结构和记者的表达模式可以通过显微技术进行可视化。

Tags

Paraffin EmbeddingThin SectioningMicrobial Colony BiofilmsMicroscopic AnalysisGene ExpressionFluorescent ProteinBiofilm ArchitectureExopolymeric SubstancesAgar Tryptone SolutionP. AeruginosaColony BiofilmsEmbedding CassetteFixativePBSMicroscopy

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