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マウス単球由来樹状細胞とその後続の in Vitro活性化腫瘍免疫複合体との隔離のプロトコル
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
DOI:

11:48 min

May 31, 2018

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Chapters

  • 00:04Title
  • 01:11Isolation of Tumor Associated Monocyte-derived DC
  • 04:07Isolation of Monocyte-derived DC from Peripheral Blood
  • 06:54Preparation of Tumor-IgG Immune Complexes Followed by Activation of MoDC with Tumor-IgG
  • 08:58Results: Isolation and Analysis of the Activation of MoDC with the Tumor Immune Complex
  • 11:02Conclusion

Summary

Automatic Translation

単球由来 DC (研) は危険性関連分子のマイナーな量を感じることができるし、簡単にプライミング、したがって。血液、腫瘍、彼らの早期活性化を避けるために考慮すべき重要注意事項を強調しながら免疫複合体とその活性化から研の隔離のための詳しいプロトコルを提供します。

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