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尺寸事项:新生隐球菌中胶囊直径的测定
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Immunology and Infection
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JoVE Journal Immunology and Infection
Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
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尺寸事项:新生隐球菌中胶囊直径的测定

DOI:
10.3791/57171-v

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08:24 min

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February 27, 2018

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, , , , , ,
1Department of Biology,Middle Tennessee State University, 2DRVision Technologies

Chapters

  • 00:04Title
  • 00:50Capsule Induction and Yeast Cell Staining
  • 03:14Image Acquisition
  • 04:27Capsule Measurement
  • 06:33Results: Representative Capsule Measurement
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

多糖胶囊是新生隐球菌的主要毒力因子, 其大小与菌株的毒力密切相关。胶囊直径测量用于表型检测和测量疗效。本文提出了一种标准的胶囊诱导方法, 并比较了两种染色法和测量直径法。

Tags

Cryptococcus NeoformansCapsule DiameterCapsule StainingIndia InkCalcofluor-whiteAlexa Fluor 488Capsule MeasurementVirulenceAutomated Analysis

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