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小鼠骨髓胰岛移植的一种方法
JoVE Journal
Biology
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JoVE Journal
Biology
A Method for Islet Transplantation to the Omentum in Mouse
Please note that all translations are automatically generated.
Click here for the English version.
小鼠骨髓胰岛移植的一种方法
DOI:
10.3791/57160-v
•
06:25 min
•
January 07, 2019
•
Ying Lu*
1
,
Shangyou Zou*
1
,
Suzanne Bertera*
2
,
Rita Bottino
,
David K.C. Cooper
,
Zhengzhao Liu
,
Yi Huang
,
Chengjun Wang
,
Chungu Hong
,
Tian He
,
Hancheng Zhang
,
Qin Huo
,
Hongxing Fu
,
Zhiming Cai
,
Lisha Mou
1
Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People’s Hospital
,
First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, Shenzhen University Health Science Center
,
2
Institute for Cellular Therapeutics
,
Allegheny-Singer Research Institute
,
3
Xenotransplantation Program/Department of Surgery
,
The University of Alabama at Birmingham
,
4
College of Life Science and Oceanography
,
Shenzhen University
,
5
School of Pharmcy
,
Wenzhou Medical College
Chapters
00:04
Title
00:46
Islet Transplantation to the Omentum
04:58
Results: Histological Staining of Retrieved Graft
05:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
介绍了一种小鼠胰岛外移植的方法。分离的胰岛与水凝胶混合, 混合物放入糖尿病小鼠的小袋中。然后对血糖进行监测, 并进行免疫组织化学分析。
Tags
Islet Transplantation
Omentum
Mouse
Type I Diabetes
Hydrogel
Anesthesia
Surgery
Sterile Technique
Centrifugation
Pipette
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