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Journal
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Biology
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带谱系特异标记的间充质细胞脂肪分化电位的可视化与量化
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
Please note that all translations are automatically generated.
Click here for the English version.
带谱系特异标记的间充质细胞脂肪分化电位的可视化与量化
DOI:
10.3791/57153-v
•
13:26 min
•
March 31, 2018
•
Jennifer Eom
,
Vaughan Feisst
,
Louis Ranjard
,
Kerry Loomes
,
Tanvi Damani
,
Victoria Jackson-Patel
3
,
Michelle Locke
5
,
Hilary Sheppard
,
Pritika Narayan
6
,
P. Rod Dunbar
7
1
School of Biological Sciences
,
The University of Auckland
,
2
Research School of Biology
,
The Australian National University
,
3
Auckland Cancer Society Research Centre, Faculty of Medical and Health Sciences
,
The University of Auckland
,
4
Department of Plastic Surgery
,
Counties Manukau District Health Board
,
5
Department of Surgery, Faculty of Medical and Health Sciences
,
The University of Auckland
,
6
Biomedical Imaging Research Unit, Faculty of Medical and Health Sciences
,
The University of Auckland
,
7
Maurice Wilkins Centre
,
The University of Auckland
Chapters
00:00
Title
01:55
Inducing the differentiation of human adipose derived stromal cells
03:36
Staining for differentiation
05:53
Imaging cells using high content screening microscope
08:23
Image Analysis: Cell Scoring
09:43
Image Analysis: Transfluor
10:43
Representative results
Summary
Automatic Translation
English (Original)
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Automatic Translation
传统的评价脂肪分化的方法既便宜又容易使用, 但并不特定于基因表达的变化。我们已经开发出一种用谱系特异标记将间充质细胞分化为成熟脂肪的实验。这种检测方法在基础研究和临床医学中有不同的应用。
Tags
Mesenchymal Cell
Adipogenic Differentiation
Lineage-specific Marker
Fatty Acid Binding Protein 4
Stromal Cells
High-content Screening
Immunofluorescent Microscopy
Image Analysis
Oil Red O
Gene Expression
Drug Screening
96-well Plate
Confluence
Differentiation Medium
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