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Journal
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Cancer Research
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干血和血清斑点作为样品贮存的有用工具, 用于评估肿瘤生物标志物
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Dried Blood and Serum Spots As A Useful Tool for Sample Storage to Evaluate Cancer Biomarkers
Please note that all translations are automatically generated.
Click here for the English version.
干血和血清斑点作为样品贮存的有用工具, 用于评估肿瘤生物标志物
DOI:
10.3791/57113-v
•
08:44 min
•
June 11, 2018
•
Laura Mercatali
,
Patrizia Serra
,
Giacomo Miserocchi
,
Chiara Spadazzi
,
Chiara Liverani
,
Alessandro De Vita
,
Giorgia Marisi
,
Alberto Bongiovanni
,
Federica Recine
,
Akwilina Pangan
,
Nestory Masalu
,
Toni Ibrahim
,
Dino Amadori
1
Osteoncology and Rare Tumors Center
,
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy
,
2
Unit of Biostatistics and Clinical Trials
,
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy
,
3
Biosciences Laboratory
,
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy
,
4
Department of Oncology
,
Bugando Medical Center, Mwanza, Tanzania
Chapters
00:04
Title
01:20
Blood Storage in Dried Blood Spots (DBS)
02:20
DNA Extraction Starting from DBS
04:51
Serum Storage for Dried Serum Spots (DSS)
06:02
ELISA Assay Starting from DSS
06:47
Results: Successful Storage of High Quality Blood and Serum at Room Temperature
07:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种简单有效的方法, 用于存储外周血和血清/血浆, 用于下游分析, 如单核苷酸多态性 (SNP) 评价和 ELISA 检测。
Tags
Dried Blood Spots
Serum Spots
Sample Storage
Cancer Biomarkers
Translational Research
Biomarker Analysis
QPCR
ELISA
Diagnosis
Infectious Diseases
Prenatal Diseases
HIV
Blood Sample Collection
DNA Extraction
Proteinase K
Lysis Buffer
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