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从果蝇幼虫中提取包囊微生物感染及分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis
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从果蝇幼虫中提取包囊微生物感染及分析

DOI:
10.3791/57077-v

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08:54 min

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May 24, 2018

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, ,
1School of Molecular Biosciences, College of Veterinary Medicine,Washington State University, 2Integrative Physiology and Neuroscience, College of Veterinary Medicine,Washington State University

Chapters

  • 00:05Title
  • 00:45Ex Vivo Infection
  • 01:48Hemolymph Extraction
  • 03:11In Vivo Infection
  • 04:08Hemolymph Extraction and Hemocyte Plating
  • 04:35Confocal Imaging
  • 05:443D Model Reconstruction
  • 06:58Results: Dynamic Visualization of Drosophila Hemocytes
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

该方法展示了如何用三维 (3D) 模型来可视化病原体侵入昆虫细胞。包囊从果蝇幼虫感染病毒或细菌病原体, 无论是体内或体内。感染的包囊然后固定和染色的成像与共聚焦显微镜和随后的3D 细胞重建。

Tags

HemocytesDrosophila MelanogasterMicrobial InfectionImmune ResponseEx Vivo InfectionConfocal Microscopy3D Model ReconstructionCapillary TubeNanoinjectorHemolymphHemocyte IsolationDrosophila Hemocyte Isolating Medium (DHIM)AnesthesiaGrape Juice Agar PlateYeast Paste

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