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Évaluation de la cytotoxique et effets immunomodulateurs de Substances dans des tranches de poumon humain de coupe de précision
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1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery,Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL),Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology,RWTH Aachen University, 6Institute for Immunology,Hannover Medical School
Chapters
- 00:05Title
- 01:26General Preparation of Human PCLS and Subsequent Exposure to Chemicals
- 04:28Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay
- 06:01LDH Assay
- 07:29Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)
- 09:49Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS
- 10:59Conclusion
Compte tenu du principe des 3 r, modèles respiratoires comme des alternatives aux études sur les animaux évoluent. Surtout pour l’évaluation des risques des substances respiratoires, il y a un manque d’essais appropriés. Nous décrivons ici l’utilisation de tranches de poumon humain de coupe de précision pour l’évaluation des substances véhiculées.
