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Cancer Research
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時間経過、ラベル無料、定量的相休眠とアクティブなひと癌細胞のイメージング研究
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
時間経過、ラベル無料、定量的相休眠とアクティブなひと癌細胞のイメージング研究
DOI:
10.3791/57035-v
•
12:48 min
•
February 16, 2018
•
Jing Huang*
1,2
,
Peng Guo*
1,2
,
Marsha A. Moses
2
1
Vascular Biology Program
,
Boston Children’s Hospital
,
2
Department of Surgery
,
Harvard Medical School and Boston Children’s Hospital
Chapters
00:04
Title
00:58
Cell Preparation
02:35
Cell Preparation: Splitting KHOS-A and KHOS-N Cells
03:50
Cell Preparation: Seeding KHOS-A and KHOS-N Cells for QPI
04:30
Quantitative Phase Image Acquisition: Cover Slip Set-up
05:19
Quantitative Phase Imaging
07:03
Analyzing Cell Morphology
08:20
Analyzing Cell Proliferation
08:54
Analyzing Cell Motion
10:16
Results: Quantiative Phase Imaging of Dormant and Active Human Cancer Cells
11:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
休止状態とアクティブな癌細胞の表現型は、定量的な位相画像を用いた特徴付けられました。細胞の増殖、移行、および形態の試金を統合し、1 つの単純な方法で分析します。
Tags
Time-lapse
Label-free
Quantitative Phase Imaging
Dormant Cancer Cells
Active Cancer Cells
Angiogenic Phenotype
Non-angiogenic Phenotype
Osteosarcoma Cells
Cell Morphology
Cell Proliferation
Cell Motility
Tumor Dormancy
Endogenic Switch
Multicellular Parameters
Cell Area
Cell Thickness
Cell Volume
Proliferation Rate
Doubling Time
Motility Speed
Migration
Cell Culture
Cell Passaging
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