JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
利用受刺激的排放损耗 (STED) 显微镜可视化肌动蛋白和微管骨架在 B 细胞免疫突触中的应用
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
利用受刺激的排放损耗 (STED) 显微镜可视化肌动蛋白和微管骨架在 B 细胞免疫突触中的应用
DOI:
10.3791/57028-v
•
11:00 min
•
April 09, 2018
•
Jia C. Wang
,
Madison Bolger-Munro
,
Michael R. Gold
1
Department of Microbiology and Immunology
,
University of British Columbia
Chapters
00:05
Title
01:18
GFP-Fusion Protein Expression and Anti-IgG Antibody Glass Coverslip Coating
03:14
Cell Spreading, Fixing, and Immunostaining
05:44
Stimulated Emission Depletion Microscope Imaging
08:02
Results: Representative STED Imaging
09:50
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们提出了一个使用 STED 显微术的协议, 同时图像肌动蛋白结构, 微管和微管加端结合蛋白的 b 细胞已扩散到盖玻片的抗体涂层的 b 细胞受体, 一个模型的初始阶段免疫突触形成。
Tags
Actin
Microtubule
Cytoskeleton
B-cell
Immune Synapse
STED Microscopy
Cytoskeleton Organization
Cytoskeleton Reorganization
Immune Cell Activation
Fluorophores
A20 B-lymphoma Cells
Transfection
Goat Anti-mouse Immunoglobulin G Antibody
Coverslips
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
星状细胞的七个步骤
测量细菌感染小鼠和免疫反应
李斯特菌</em
寄生蜂简介<em>果蝇</em>和Antiparasite的免疫反应的
活体成像的鼠标使用2 - 光子显微镜的胸腺
使用一个调查益生素对肺炎球菌定植的影响<em>体外</em>粘附试验
免疫突触由双色时间选通受激发射损耗(STED)纳米显微可视化
在
MyD88的介导的细胞免疫应答西尼罗河病毒变异株感染
体外
分析
影像性流式细胞仪对人体免疫突触的定性和定量分析
利用共焦显微镜可视化巨噬细胞胞外陷阱
脂肪组织中的血管和神经纤维的共染色
Read Article