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Cancer Research
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流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访
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JoVE Journal
Cancer Research
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Please note that all translations are automatically generated.
Click here for the English version.
流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访
DOI:
10.3791/56976-v
•
09:01 min
•
March 26, 2018
•
Thomas Boyer
2
,
Fanny Gonzales
3
,
Adriana Plesa
,
Pauline Peyrouze
,
Adeline Barthelemy
,
Soizic Guihard
,
Bruno Quesnel
5
,
Christophe Roumier
2
,
Claude Preudhomme
2
,
Meyling Cheok
1
Laboratoire d’hématologie
,
CHU Lille
,
2
Univ. Lille, Inserm, CHU Lille, UMR-S 1172 – JPArc – Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer
,
3
Service d’hématologie pédiatrique, hôpital Jeanne de Flandre
,
CHU Lille
,
4
Laboratoire d’hématologie CH Lyon Sud
,
5
Service des Maladies du Sang, hôpital Claude Huriez
,
CHU Lille
Chapters
00:04
Title
00:58
Sample Preparation and Lysis of Red Blood Cells
02:26
Patient Derived Xenografts and Staining
04:50
Flow Cytometry and Gating Strategy
06:14
Results: Parametric Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia
07:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
通过流式细胞术, 我们勾勒出一种检测白血病干细胞标记物同时表达的协议。我们展示了如何量化三个祖群体和一个假定的多项种群的成熟程度。我们证实了这些人群存在于相应的病人衍生的移植。
Tags
Flow Cytometry
Leukemia Stem Cells
Acute Myeloid Leukemia
Patient-derived Xenografts
Diagnosis
Follow-up
Mononuclear Cells
Density Gradient
Red Blood Cell Lysis
NSG Mouse
Xenograft
Submandibular Blood Collection
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