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催吐剂节间培养中内源生长素和细胞分裂细胞的量化
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Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
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催吐剂节间培养中内源生长素和细胞分裂细胞的量化

DOI:
10.3791/56902-v

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07:57 min

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March 15, 2018

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, ,
1Graduate School of Life Sciences,Toyo University

Chapters

  • 00:05Title
  • 00:51Growth Conditions to Induce Adventitious Shoots of Ipecac
  • 01:46Extraction and Purification of Phytohormones
  • 04:46LC-MS/MS Analysis of IAA and CKs
  • 05:41Results: IAA and CK Levels in Adventitious Shoots Change During Five Weeks of Culture
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

无激素治疗的催吐剂茎段可诱发不定芽。为了评价不定芽形成过程中的激素动态, 我们用 LC-ms/ms 测定了茎段内源性生长素和细胞分裂素。

Tags

IpecacInternode CultureEndogenous AuxinEndogenous CytokininPhytohormone QuantificationAdventitious Shoot FormationB5 MediumZirconia BeadAcetonitrile ExtractionHPLC-MS/MS

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