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用便携式实时 PCR 系统进行土壤载体 Phytopathogens 的现场分子检测
JoVE Journal
Environment
Author Produced
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JoVE Journal
Environment
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Please note that all translations are automatically generated.
Click here for the English version.
用便携式实时 PCR 系统进行土壤载体 Phytopathogens 的现场分子检测
DOI:
10.3791/56891-v
•
14:15 min
•
February 23, 2018
•
Joseph B. DeShields
,
Rachel A. Bomberger
,
James W. Woodhall
,
David L. Wheeler
,
Natalia Moroz
,
Dennis A. Johnson
,
Kiwamu Tanaka
1
Department of Plant Pathology
,
Washington State University
,
2
Parma Research and Extension Center
,
University of Idaho
Chapters
00:00
Title
01:06
Magnetic bead-based DNA extraction
02:06
Soil lysis
11:44
Representative Results
13:24
Discussion
13:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
快速准确地检测植物病原体, 特别是土壤传播病原体, 对于防止田间植物病害进一步接种生产和增殖至关重要。采用便携式实时 PCR 检测系统开发的方法, 能够在野外条件下进行现场诊断。
Tags
On-site Detection
Soil-borne Phytopathogens
Portable Real-time PCR
Magnetic Bead-based DNA Extraction
Potato Pathogens
Spongospora Subterranea
Field Diagnosis
Plant Pathogen Detection
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