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Journal
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Immunology and Infection
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制备光学质量玻璃表面研究巨噬细胞融合
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
Please note that all translations are automatically generated.
Click here for the English version.
制备光学质量玻璃表面研究巨噬细胞融合
DOI:
10.3791/56866-v
•
08:50 min
•
March 14, 2018
•
James J. Faust
2
,
Wayne Christenson
4,5
,
Kyle Doudrick
,
John Heddleston
,
Teng-Leong Chew
,
Marko Lampe
,
Arnat Balabiyev
2
,
Robert Ros
4,5
,
Tatiana P. Ugarova
2
1
Center for Metabolic and Vascular Biology
,
Mayo Clinic
,
2
Molecular and Cellular Biosciences, School of Life Sciences
,
Arizona State University
,
3
Department of Physics
,
Arizona State University
,
4
Center for Biological Physics
,
Arizona State University
,
5
Biodesign Institute
,
Arizona State University
,
6
Department of Civil and Environmental Engineering and Earth Sciences
,
University of Notre Dame
,
7
Advanced Imaging Center
,
HHMI Janelia Research Campus
,
8
Advanced Light Microscopy Facility
,
European Molecular Biology Laboratory
Chapters
00:05
Title
00:47
Preparing Acid-cleaned Cover Glass
01:59
Preparing of Fusogenic Optical-quality Surfaces
02:52
Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
03:58
Fabricating Glass Bottom Dishes
04:55
Collecting Thioglycollate-elicited Macrophages
06:15
Results: Macrophage Fusion on Glass Surfaces
07:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了光学质量玻璃表面的制备, 这些化合物含有长链碳氢化合物, 可用于监测活体标本的巨噬细胞融合, 并能实现固定标本的超分辨显微术。.
Tags
Optical Glass
Macrophage Fusion
Microscopy
Acid Cleaning
Paraffin Coating
Gold TEM Grid
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