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Metabole Mapping: Kwantitatieve enzym cytochemie en histochemie te bepalen van de activiteit van Dehydrogenases in cellen en weefsels
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal Immunology and Infection
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
DOI:

08:36 min

May 26, 2018

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Chapters

  • 00:00Title
  • 00:50Preparing Reaction Buffer
  • 03:50Applying Incubation Medium to Tissue Sections
  • 05:32Washing the Microscopy Slides
  • 06:33Enclose the Tissue Sections on Microscopy Slides
  • 07:13Representative Results
  • 08:14Conclusion

Summary

Automatic Translation

Hier presenteren we een protocol dat kan worden gebruikt om microscopisch visualiseren en kwantificeren van de activiteit van dehydrogenases in cellen en weefsels en kinetiek, functie en subcellular localisatie.

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