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Journal
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Immunology and Infection
/
一种新
的体外
创面愈合试验评价细胞迁移
JoVE Journal
Immunology and Infection
Author Produced
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Immunology and Infection
A Novel
In Vitro
Wound Healing Assay to Evaluate Cell Migration
Please note that all translations are automatically generated.
Click here for the English version.
一种新
的体外
创面愈合试验评价细胞迁移
DOI:
10.3791/56825-v
•
08:55 min
•
March 17, 2018
•
Floriana Cappiello
,
Bruno Casciaro
,
Maria Luisa Mangoni
1
Department of Biochemical Sciences
,
Sapienza University of Rome
Chapters
00:00
Title
01:28
Cell Preparation
03:34
Cell Seeding in the Culture Inserts
04:30
Pseudo-wound Healing Assay
05:52
Image Analysis
06:53
Representative Results
07:34
Effects of Inhibitors on Cells Migration
08:33
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出一个评估肽对支气管上皮细胞迁移的影响的协议。这种方法允许快速和高度重复获取的数量数据的速度的细胞迁移和伤口闭合。
Tags
Cell Migration
Wound Healing
In Vitro Assay
Immunomodulatory Molecules
Antimicropeptides
Cell Monolayer
Pseudo-wound
Image Analysis
Bronchial Epithelial Cells
Epidermal Growth Factor Receptor
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