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基于细胞荧光检测平台的γ-分泌介导的淀粉样前体蛋白和缺口裂解的定量测定
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Neuroscience
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JoVE Journal Neuroscience
Quantitative Measurement of γ-Secretase-mediated Amyloid Precursor Protein and Notch Cleavage in Cell-based Luciferase Reporter Assay Platforms
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基于细胞荧光检测平台的γ-分泌介导的淀粉样前体蛋白和缺口裂解的定量测定

DOI:
10.3791/56795-v

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06:40 min

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January 25, 2018

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, , , , , ,
1Laboratory of Molecular Neurobiology, Institute of Cellular and Organismic Biology,Academia Sinica

Chapters

  • 00:05Title
  • 01:17Measurement of Luciferase Reporter Signals, which Correspond to γ-Secretase Cleavage of APP-C99 (CG cells) or NΔE (NG cells)
  • 03:42Quantification of γ-Secretase Activity
  • 04:40Results: Treatments with CL-387, 785 can Differentially Promote the Processing of APP-C99 by γ-Secretase without Affecting Notch Cleavage
  • 06:05Conclusion

Summary

Automatic Translation

Automatic Translation

我们已经成功地生成了两个基片特定的γ-分泌检测。这两种基于细胞的检测方法都被设计成通过萤火虫荧光记者的输出来量化γ-分泌酶的活性。

Tags

Gamma-secretaseAmyloid Precursor ProteinNotch CleavageCell-based Luciferase Reporter AssayAlzheimer’s DiseaseGamma-secretase ModulatorsSubstrate SelectivityNeuroblastomaNG CellsCG CellsTetracyclineDMSOCL-387785DAPTErbB1/ErbB2 InhibitorsLuciferase AssayLuminescence Microplate Reader

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