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Journal
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Immunology and Infection
/
全自动微流控装置在
白色念珠菌
生物膜形成中的可视化
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Visualization of Biofilm Formation in
Candida albicans
Using an Automated Microfluidic Device
Please note that all translations are automatically generated.
Click here for the English version.
全自动微流控装置在
白色念珠菌
生物膜形成中的可视化
DOI:
10.3791/56743-v
•
11:14 min
•
December 14, 2017
•
Megha Gulati
,
Craig L. Ennis
,
Diana L. Rodriguez
,
Clarissa J. Nobile
1
Department of Molecular and Cell Biology, School of Natural Sciences
,
University of California, Merced
Chapters
00:05
Title
01:02
Preparation of Microfluidic Channels of the Microfluidic Plate
02:08
Candida albicans
Biofilm Formation in the Microfluidic System
04:06
Setting Up the Stage Positions for the Microfluidic Experiment
06:01
Microfluidic Experiment Image Acquisition
07:38
Analyzing the Results
09:07
Results: Drug Exposure and Genetic Mutations Can Alter Biofilm Formation
10:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了在宿主生理条件下, 使用可定制的自动微流控装置来可视化
白色念珠菌
中的生物膜形成。
Tags
Biofilm Formation
Candida Albicans
Microfluidic Device
Real-time Visualization
Antimicrobial Agents
Genetic Mutants
High-throughput Screening
YPD Medium
Temperature Control
Shear Stress
Constant Flow Mode
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