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Journal
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Biology
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体外
相撲 E3 リガーゼ活性を研究する sumo 化アッセイ
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In Vitro
SUMOylation Assay to Study SUMO E3 Ligase Activity
Please note that all translations are automatically generated.
Click here for the English version.
体外
相撲 E3 リガーゼ活性を研究する sumo 化アッセイ
DOI:
10.3791/56629-v
•
09:45 min
•
January 29, 2018
•
Wan-Shan Yang
,
Mel Campbell
,
Hsing-Jien Kung
3,4,5
,
Pei-Ching Chang
6
1
Institute of Microbiology and Immunology
,
National Yang-Ming University
,
2
UC Davis Cancer Center
,
University of California, Davis
,
3
Department of Biochemistry and Molecular Medicine
,
University of California, Davis
,
4
Institute for Translational Medicine, College of Medical Science and Technology
,
Taipei Medical University
,
5
Division of Molecular and Genomic Medicine
,
National Health Research Institutes
,
6
Center for Infectious Disease and Cancer Research
,
Kaohsiung Medical University
Chapters
00:05
Title
00:39
Purification of K-bZIP
03:26
SUMOylation Assay
07:15
Results: Use of
In Vitro
SUMOylation Assay to Study the Role of SUMO E3 Ligase in p53 SUMOylation Using Optimal Ubc9 Enzyme Concentration
08:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ユビキチンリ ガーゼとは異なりいくつかの相撲 E3 リガーゼが同定されています。この変更された
体外
sumo 化プロトコル
の in vitro
再構成法による新規相撲 E3 リガーゼを識別することです。
Tags
In Vitro SUMOylation
SUMO E3 Ligase Activity
SUMO E3 Ligase Identification
SUMO Isoform Specificity
Protein Purification
Magnetic Bead Pulldown
SDS-PAGE
Protein Quantification
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