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ΓH2AX、53BP1 形成と DNA 二重鎖切断の修復を分析するための蛍光顕微鏡観察
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Cancer Research
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JoVE Journal
Cancer Research
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks
Please note that all translations are automatically generated.
Click here for the English version.
ΓH2AX、53BP1 形成と DNA 二重鎖切断の修復を分析するための蛍光顕微鏡観察
DOI:
10.3791/56617-v
•
10:47 min
•
November 03, 2017
•
Henning D. Popp
,
Susanne Brendel
,
Wolf-Karsten Hofmann
,
Alice Fabarius
1
Department of Hematology and Oncology
,
Medical Faculty Mannheim of the Heidelberg University
Chapters
00:05
Title
00:52
Material Preparation
03:28
Mononuclear Cells Isolation by Density Gradient Centrifugation
05:26
Cytospin Preparation, γH2AX and 53BP1 Immunofluorescence Staining, and Analysis
08:46
Results: Clearly Visible Foci and DNA Repair
10:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
この原稿は、γH2AX、53BP1 の蛍光顕微鏡による DNA 二重鎖切断の分析のためのプロトコルを提供します。
Tags
Immunofluorescence Microscopy
γH2AX
53BP1
DNA Double-strand Breaks
Cancer Research
Genetic Instability
Cell Fixation
Permeabilization
Blocking Solution
Blood Sample
Bone Marrow Sample
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