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Immunology and Infection
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重组原型泡沫病毒整合纯化过程中核酸污染的检测与去除
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
Please note that all translations are automatically generated.
Click here for the English version.
重组原型泡沫病毒整合纯化过程中核酸污染的检测与去除
DOI:
10.3791/56605-v
•
07:50 min
•
December 08, 2017
•
Miguel A. Lopez Jr.
,
Randi M. Mackler
,
Matthew P. Altman
,
Kristine E. Yoder
1
Department of Cancer Biology and Genetics
,
The Ohio State University College of Medicine
Chapters
00:05
Title
00:35
Nickel Affinity Chromatography
03:30
Heparin Affinity Chromatography
04:28
Nuclease Assay
06:46
Results: Nuclease Assay of Heparin Sepharose Fractions
07:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
重组原型泡沫病毒整合蛋白经常被细菌核酸在纯化过程中污染。这种方法识别核酸污染, 并将其从酶的最终制备中除去。
Tags
Nuclease Contamination
Protein Purification
Recombinant Prototype Foamy Virus Integrase
E. Coli
Sonication
Ultracentrifugation
FPLC
Nickel-charged Resin
SDS-PAGE
UV Absorbance
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