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拟南芥种子高通量灭菌的标准化方法
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
Please note that all translations are automatically generated.
Click here for the English version.
拟南芥种子高通量灭菌的标准化方法
DOI:
10.3791/56587-v
•
08:13 min
•
October 17, 2017
•
Benson E. Lindsey III*
1
,
Luz Rivero*
1
,
Chistopher S. Calhoun
,
Erich Grotewold
2
,
Jelena Brkljacic
1
Arabidopsis Biological Resource Center, Center for Applied Plant Sciences
,
The Ohio State University
,
2
Department of Molecular Genetics, Center for Applied Plant Sciences
,
The Ohio State University
Chapters
00:05
Title
00:41
Sterilization of Arabidopsis Seeds with Bleach
02:14
Sterilization of Arabidopsis Seed with Chlorine Gas
05:01
Seed Plating and Evaluating Germination
05:59
Results: Effects of Bleach or Chlorine Gas Sterilization on Seed Germination
07:28
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究的目的是确定漂白和氯气灭菌对不育培养基上的拟南芥种子萌发的影响。为防止微生物污染物的生长, 在满足种子存活的同时, 制定了优化的灭菌规程。
Tags
Arabidopsis Seeds
Seed Sterilization
High-throughput
Bleach Solution
TWEEN 20
Laminar Flow Hood
Chlorine Gas
Hydrochloric Acid
Germination Rate
Genomics
Proteomics
Metabolomics
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