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Cancer Research
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上に移動: シンプルかつ柔軟な
In Vitro
三次元侵略試金のプロトコル
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Moving Upwards: A Simple and Flexible
In Vitro
Three-dimensional Invasion Assay Protocol
Please note that all translations are automatically generated.
Click here for the English version.
上に移動: シンプルかつ柔軟な
In Vitro
三次元侵略試金のプロトコル
DOI:
10.3791/56568-v
•
06:14 min
•
March 12, 2018
•
Tanner J. McArdle
,
Brenda M. Ogle
2,3,4,5
,
Felicite K. Noubissi
1
Department of Biomedical Engineering
,
University of Minnesota – Twin Cities
,
2
Stem Cell Institute
,
University of Minnesota – Twin Cities
,
3
Masonic Cancer Center
,
University of Minnesota – Twin Cities
,
4
Lillehei Heart Institute
,
University of Minnesota – Twin Cities
,
5
Institute for Engineering in Medicine
,
University of Minnesota – Twin Cities
,
6
Department of Biology
,
Jackson State University
Chapters
00:05
Title
01:11
Culture Plate, Collagen Gel Preparation, and Cell Imaging
03:33
Results: Representative Inverted Vertical Invasion Assay Analyses
04:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルでは、細胞の微小環境を維持しながら三次元の設定のセルの移行と侵略の機能を定量化に使用できる反転垂直浸潤能の測定法について説明します。この試金は稀および/または敏感な細胞に適しています。
Tags
3D Invasion Assay
In Vitro Invasion Assay
Cell Migration
Cell Invasion
Cancer Research
Metastasis
Collagen Gel
Cell Culture
DAPI
Paraformaldehyde
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