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Immunology and Infection
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饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
Please note that all translations are automatically generated.
Click here for the English version.
饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡
DOI:
10.3791/56535-v
•
08:26 min
•
October 31, 2017
•
Yuwen Zhang
,
Jiaqing Hao
,
Yanwen Sun
,
Bing Li
1
Department of Microbiology and Immunology
,
University of Louisville
Chapters
00:05
Title
00:59
Preparation of Mouse Bone Marrow Derived Macrophages (BMDMs) and BSA-fatty Acid Conjugate
04:31
Saturated Fatty Acids Induce Cell Death of Mouse Bone Marrow Derived Macrophages
06:04
Intracellular Ceramide Staining
07:03
Results: Fatty Acid-induced Macrophage Cell Death and Ceramide Production
07:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文阐述了一种从骨髓细胞中提取小鼠原发巨噬蛋白的方法, 并提出了制备 BSA-脂肪酸共轭物的简便方法。然后我们证明饱和脂肪酸能诱导巨噬细胞死亡, 这种细胞死亡与神经酰胺水平的细胞积累呈正相关。
Tags
Saturated Fatty Acids
Macrophages
Ceramides
Cell Death
Metabolic Inflammation
BSA Fatty Acid Conjugates
Bone Marrow Cells
Red Blood Cell Lysis
RPMI 1640 Medium
Macrophage Differentiation
M-CSF
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